While gas chromatography is one of the most common methods, forensic technicians may also choose high-performance liquid chromatography, planar chromatography, or thin-layer chromatography. Before we dive into the different types of chromatography for forensic science, we must understand how chromatography works. In general, chromatography is the process of separating a mixture into its various components so that they can be easily identified. Each type of chromatography involves a mobile phase passing through a stationary phase to separate the compound.
The stationary phase may be housed within a column, such as in gas chromatography or be a simple plane, such as in planar chromatography. A mixture or compound is injected into a solvent and is then passed through the stationary phase. This process allows researchers to measure retention time and identify components of a mixture. How does chromatography apply to forensic science?
As one of the most common methods, gas chromatography GC has several uses within the forensic process. This type of chromatography uses gas as the mobile phase to move the injected substance toward the stationary phase in order to analyze the mixture by measuring their retention time. This helps forensic lab technicians identify several factors.
For example, one of the most common uses of gas chromatography in forensics is for identifying and determining a pathology conclusion, such as fluids present in a body, analyzing blood samples or identifying poisons present. In addition, these machines can help forensic scientists investigate crimes, such as arson, by testing different compounds to find flammable liquids.
Instead of using gas as the mobile phase as in gas chromatography, this method of chromatography uses a liquid mobile phase. High-performance liquid chromatography HPLC machines push substances through using a high-pressure pump. These machines help forensic scientists analyze volatile substances such as gunpowder residue, fibers, and toxins. One of its most common uses is to determine materials used in explosives. The applications range from a simple verification of the purity of a given compound to the quantitative determination of the components of a mixture.
Chromatography is based on the principle where molecules in mixture applied onto the surface or into the solid, and fluid stationary phase stable phase is separating from each other while moving with the aid of a mobile phase. Paper chromatography is a method for separating dissolved substances from one another. It is often used when the dissolved substances are coloured, such as inks, food colourings and plant dyes.
Applications of chromatography. Other applications of the tech include compound purification and crystal production. Principle of Centrifugation: When a mixture is rotated very fast, the denser particles are forced to go to the bottom of the centrifuge and the lighter particles stay at the top. Begin typing your search term above and press enter to search. Press ESC to cancel. Skip to content Home Dissertation How do forensic scientists use chromatography to solve crimes?
Ben Davis February 24, How do forensic scientists use chromatography to solve crimes? This, in turn, will have contributed to an increasing interest in forensic science and forensic chemistry as a career.
With pure chemistry courses sadly in decline, some institutions in the UK and elsewhere have converted chemistry degrees into forensic chemistry ones or added an option in the subject.
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